Protein Profiling of Pollen–Pistil Interactions in Almond (Prunus dulcis) and Identification of a Transcription Regulator Presumably Involved in Self-Incompatibility

The cultivated almond displays a gametophytic self-incompatibility system, which avoids self-fertilization, and it is controlled by multi-allelic locus (S-locus) containing two genes specifically expressed in pistil (S-RNase) pollen (SFB). Studies on almonds with the same S-haplotype but different phenotype pointed to existence of unknown components this system explain its functioning. increase knowledge reproductive barrier would allow better management fruit production germplasm selection. This work proposes candidates identifying differentially proteins (DEPs) after compatible incompatible pollen–pistil interactions incompatibility using iTRAQ 2D-nano-LC ESI/MSMS analyses. protein quantitation analysis revealed 895 DEPs, were grouped into functional categories. largest group was “metabolic proteins”, followed “stress resistance defense higher up-regulation pollination. identity certain such as Thaumatin, LRR receptors, kinase pathogenesis related PR-4, indicated that some could have bases host–parasite interactions. Furthermore, additional RT-qPCR transcription regulator GLABROUS1 enhancer-binding protein-like (GEBPL) be involved almond.